New Science Advances Paper by the Aebi Lab
Mechanistic reconstruction of glycoprotein secretion through monitoring of intracellular N-glycan processing
Ilaria Arigoni-Affolter, Ernesto Scibona, Chia-Wei Lin, David Brühlmann, Jonathan Souquet, Hervé Broly and Markus Aebi
Science Advances 27 Nov 2019, DOI: 10.1126/sciadv.aax8930
N-linked glycosylation plays a fundamental role in determining the thermodynamic stability of proteins and is involved in multiple key biological processes. The mechanistic understanding of the intracellular machinery responsible for the stepwise biosynthesis of N-glycans is still incomplete due to limited understanding of in vivo kinetics of N-glycan processing along the secretory pathway. We present a glycoproteomics approach to monitor the processing of site-specific N-glycans in CHO cells. On the basis of a model-based analysis of structure-specific turnover rates, we provide a kinetic description of intracellular N-glycan processing along the entire secretory pathway. This approach refines and further extends the current knowledge on N-glycans biosynthesis and provides a basis to quantify alterations in the glycoprotein processing machinery.